A display screen, a pair of glasses, a display system and a playing method are provided. The display screen includes a display control module and a display panel. The display control module controls the display panel to display multiple frames of images according to multiple predetermined playing codes. Each playing code corresponds to one frame of image. Each frame of image is displayed as normal image or interference image based on different logic values of the corresponding playing code. The multiple frames of images are divided into multiple consecutive frame groups. The quantity of consecutive frames corresponding to playing codes having an identical logic value in each frame group is not larger than a predetermined value, such that a user wearing glasses matching the display screen can see the normal images displayed on the display panel and cannot see the interference images displayed on the display panel