Provided are a planographic printing plate precursor for on-press development, including: an aluminum support; an interlayer which contains a compound containing a support absorptive group and a hydrophilic group; and an image recording layer which contains an infrared absorbing agent, a polymerization initiator, a polymerizable compound, and polymer particles formed of a styrene copolymer, on the aluminum support, in which the aluminum support is an aluminum plate having an anodized film on a surface in contact with the interlayer and the anodized film has micropores extending in a depth direction from a surface in contact with the interlayer, and an average pore diameter of the micropores in the surface of the anodized film is in a range of 20 to 40 nm; a method of preparing a planographic printing plate and a planographic printing method obtained by using the planographic printing plate precursor for on-press development.