[Object] To analyze a strain of a biological sample more accurately. [Solution] Provided is an information processing device including: a setting unit configured to set at least one region of interest from one captured image constituting a dynamic image for a biological sample; an analysis object specifying unit configured to specify an analysis object for the at least one region of interest; a detection unit configured to detect a motion of the analysis object in the dynamic image; and an analysis unit configured to analyze a strain of the biological sample related to the at least one region of interest on a basis of the detected motion of the analysis object.