For an imaging method for presenting fluorophores (5) by optical excitation, spontaneous emission of fluorescence and detection of same, a single, conventional sensor (6) is used having at least two color channels, which detect an excitation light used to excite the fluorophore (5) and the fluorescence emitted by the fluorophore (5) with different sensitivities. Due to the different spectral distribution of the sensitivity of the color channels, it is possible to separate the component of the excitation light from the component of the fluorescence, in particular in a specific pixel, from one another by processing output signals of said color channels, in particular by conversion into a color space and/or by calculation of color saturation values. From this, the intensity of the fluorescence can be deduced, preferably taking account of a relative luminance measured by the color channels, even though reflected excitation light reaches the color channels, in particular in an unfiltered manner.