Invention Application
- Patent Title: CELL-FREE PROTEIN EXPRESSION USING ROLLING CIRCLE AMPLIFICATION PRODUCT
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Application No.: US16041734Application Date: 2018-07-20
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Publication No.: US20190071704A1Publication Date: 2019-03-07
- Inventor: John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
- Applicant: GENERAL ELECTRIC COMPANY
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C12N15/67 ; C12N15/63
Abstract:
Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
Public/Granted literature
- US11421259B2 Cell-free protein expression using rolling circle amplification product Public/Granted day:2022-08-23
Information query
