Variable temperature analytical instrument components are provided that can include: a conduit configured to extend between two chambers having different pressures; and a first mass about a section of the conduit, wherein the first mass is maintained at a first temperature. Variable temperature analytical instruments are provided that can include: a sample chamber configured to be operably coupled to a heat or cold source via one or more conduits; an interface component configured to engage the one or more conduits with the sample chamber, the interface comprising a conduit configured to extend between two chambers having different pressures; and a first mass about a section of the conduit, wherein the first mass is maintained at a first temperature. Methods for maintaining temperatures within a variable temperature analytical instrument are also provided. These methods can include maintaining a temperature within a conduit extending between two components of the instrument while each component is maintained at different pressures.