- 专利标题: CHIMERIC DOUBLE-STRANDED NUCLEIC ACID
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申请号: US16410803申请日: 2019-05-13
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公开(公告)号: US20190270996A1公开(公告)日: 2019-09-05
- 发明人: TAKANORI YOKOTA , Kazutaka Nishina , Satoshi Obika , Hidehiro Mizusawa
- 申请人: National University Corporation Tokyo Medical and Dental University , Osaka University
- 申请人地址: JP Tokyo JP Osaka
- 专利权人: National University Corporation Tokyo Medical and Dental University,Osaka University
- 当前专利权人: National University Corporation Tokyo Medical and Dental University,Osaka University
- 当前专利权人地址: JP Tokyo JP Osaka
- 优先权: JP2011-275488 20111216
- 主分类号: C12N15/113
- IPC分类号: C12N15/113 ; C12N15/11
摘要:
A method of reducing the level of a transcription product in a cell comprising contacting with the cell a composition comprising a double-stranded nucleic acid complex comprising a first nucleic acid strand annealed to a second nucleic acid strand, wherein: (i) the first nucleic acid strand hybridizes to the transcription product and comprises (a) a region consisting of at least 4 consecutive nucleotides that are recognized by RNase H when the strand is hybridized to the transcription product, (b) one or more nucleotide analogs located on 5′ terminal side of the region, (c) one or more nucleotide analogs located on 3′ terminal side of the region and (d) a total number of nucleotides and nucleotide analogs ranging from 8 to 35 nucleotides and (ii) the second nucleic acid strand comprises (a) nucleotides and optionally nucleotide analogs and (b) at least 4 consecutive RNA nucleotides.
公开/授权文献
- US11034955B2 Chimeric double-stranded nucleic acid 公开/授权日:2021-06-15