A cartridge for use in a tissue substitute printing system is disclosed, the cartridge may have: a material reservoir, the material reservoir storing a tissue substitute material in a flowable, in particular paste-like form; a material aperture arranged at a bottom side of the cartridge, the material aperture being fluidic coupled with the material reservoir for releasing tissue substitute material stored in the material reservoir; at least one auxiliary aperture arranged at the bottom side of the cartridge for releasing an auxiliary medium, the at least one auxiliary aperture being arranged in proximity to and fluidic separate from the material aperture. A tissue substitute printing system and a method for manufacturing a tissue substitute are also disclosed.