The present disclosure discloses a display panel, a display device and a manufacturing method of a display panel, in the field of display technology. The display panel includes a plurality of light-emitting unit groups. A light-transmitting region is between every two adjacent light-emitting unit groups. Each light-emitting unit group includes a light-shielding layer and a light-emitting unit. According to the display panel provided in the present disclosure, light emitted by the light-emitting unit can be viewed from on one side, and light emitted by the light-emitting unit cannot be emitted from the other side due to shielding by the light-shielding layer. Since the light-transmitting region exists, the other side is still transparent. Thus, the display panel can be combined with varied transparent components, which diversifies function of the display panel.