Techniques and systems for synchronized accessibility for client devices in an online conference are described. For example, a conferencing system receives presentation content and audio content as part of the online conference from a client device. The conferencing system generates sign language content by converting audio in the audio content to sign language. The conferencing system then synchronizes display of the sign language content with the presentation content in a user interface based on differences in durations of segments of the audio content from durations of corresponding segments of the sign language content. Then, the conferencing system outputs the sign language content as synchronized with the presentation content, such as to a viewer client device that requested the sign language content, or to storage for later access by viewers that request sign language content.