The disclosure provides a pixel circuit, a display panel and a driving method thereof. The pixel circuit includes a driving transistor, a threshold storage subcircuit, a threshold storage control subcircuit, a data storage subcircuit, a data writing control subcircuit, an isolation control subcircuit, a light-emitting control subcircuit and a light-emitting diode. The threshold storage control subcircuit is coupled to the threshold storage subcircuit. The threshold storage subcircuit stores the reference voltage input by the threshold storage control subcircuit, and stores a threshold voltage of the driving transistor. The data writing control subcircuit is coupled to the data storage subcircuit. The data storage subcircuit stores the data voltage input by the data writing control subcircuit. The isolation control subcircuit is coupled between the data storage subcircuit and the threshold storage subcircuit. The driving transistor is coupled to the threshold storage subcircuit and the data storage subcircuit.