A display substrate and a display device are disclosed. The display substrate (10) includes a plurality of repeating units (100), a plurality of primary signal lines (21) and an auxiliary signal line (22). Each of the repeating units (100) includes a first sub-pixel (R1), a second sub-pixel (B1) and two third sub-pixels (G1, G2). The two third sub-pixels (G1, G2) are located between two adjacent primary signal lines (21). In each of the repeating units (100), the first sub-pixel (R1) and the second sub-pixel (B1) are arranged in a first direction (X), and the two third sub-pixels (G1, G2) are arranged in a second direction (Y). The first direction (X) and the second direction (Y) are different directions. At least one auxiliary signal line (22) is disposed between the two adjacent primary signal lines (21). Two ends of the auxiliary signal line (22) are respectively connected to the two adjacent primary signal lines (21). The two third sub-pixels (G1, G2) are respectively located on two sides of the auxiliary signal line (22).