A golf tee tethering assembly for inhibiting a rubber mat tee from flying away during golf practice includes a rubber mat tee that is positionable on a rubber golfing mat to have a golf ball positioned thereon for striking. A washer is positioned around the rubber mat tee and a tether is coupled to the washer. A fastener is coupled to the tether on an opposite end of the tether from the ring. The fastener releasably engages a shaft of a golf club such that the rubber mat tee is retained on the golf club. The golf club is laid on the rubber golf mat and the rubber mat tee is positioned at a chosen spot on the rubber golf mat. Thus, the tether inhibits the rubber mat tee from flying away when the golf ball is struck from the rubber mat tee.