A portable urine detection device and method is disclosed by the invention which relates to the technical field of urine detection. The invention includes a detection device and a cloud backstage system, of which the detection device comprises a shell, a slot component, a fixed component and several test strips. The circuit board is fixed in the shell. Several LED lamp beads, a camera module, a first algorithm module, a second algorithm module, a third algorithm module and a fourth algorithm module are fixed on one surface of the circuit board. One side of the circuit board is provided a USB port; one side of the USB port penetrates one side of the shell; the other side of the shell is provided with an opening; one side of the shell is connected with a smart phone through a USB port and an OTG data line. The fixed component comprises a bottom plate. According to the invention, the problems of overlarge volume, incapability of universal use and higher cost of the existing urine detection device are solved by designing a novel urine detection structure which is designed by a corresponding algorithm module that connected with the cloud.