- 专利标题： NON-HUMAN GENE-EDITED MAMMAL, PROTEIN CRUDE EXTRACT SEPARATED FROM CONNECTIVE TISSUE OF NON-HUMAN GENE-EDITED MAMMAL, METHOD FOR PROTEIN CRUDE EXTRACT AND USES OF PROTEIN CRUDE EXTRACT
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申请号： US16646172申请日： 2017-09-25
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公开(公告)号： US20210032311A1公开(公告)日： 2021-02-04
- 发明人: Yu-Chun Wu , Pi-Hung Liao , Shih-Chieh Tsai
- 申请人： Yu-Chun Wu , Pi-Hung Liao , Shih-Chieh Tsai
- 申请人地址： TW Tainan City; TW Kaohsiung City; TW Tainan City
- 专利权人： Yu-Chun Wu,Pi-Hung Liao,Shih-Chieh Tsai
- 当前专利权人： Yu-Chun Wu,Pi-Hung Liao,Shih-Chieh Tsai
- 当前专利权人地址： TW Tainan City; TW Kaohsiung City; TW Tainan City
- 国际申请： PCT/CN2017/103194 WO 20170925
- 主分类号： C07K14/78
- IPC分类号： C07K14/78 ; A01K67/027 ; C12N15/85
摘要：
A non-human gene-edited mammal, a protein crude extract isolated from a connective tissue of the non-human gene-edited mammal, a method for preparing the protein crude extract, and uses of the protein crude extract are provided. The method includes: microinjecting a deoxyribonucleic acid sequence construct (DNA construct) containing SEQ ID NO: 4 or SEQ ID NO: 5 into a rat embryo, transplanting the rat embryo into a female rat of the same species to develop into a mature rat, and isolating a protein crude extract from a connective tissue of the mature rat. The protein crude extract includes human type I collagen and a non-native chimeric protein peptide chain.