The present disclosure relates to a display device, a display panel, and a manufacturing method thereof. The display panel includes a substrate, a driving layer, and a display layer. The substrate has an opening area, a transition area surrounding the opening area, and a display area surrounding the transition area. The driving layer is disposed on a side of the substrate and covers at least the transition area and the display area, an area of the driving layer being located in the transition area being provided with a separation groove surrounding the opening area, the separation groove including a first groove body and a second groove body sequentially communicated toward the substrate in a direction perpendicular to the substrate, and a distance between bottom ends of side walls of the first groove body being smaller than a distance between top ends of side walls of the second groove body.