- 专利标题： A METHOD FOR CREATING MALE STERILE LINE OF TOMATO THROUGH GENOME EDITING AND APPLICATION THEREOF
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申请号： US16623659申请日： 2017-11-20
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公开(公告)号： US20210095308A1公开(公告)日： 2021-04-01
- 发明人: Changbao LI , Minmin DU , Chuanyou LI , Lei DENG , Ming ZHOU , Changlong WEN
- 申请人： BEIJING ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES , Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
- 申请人地址： CN Beijing; CN Beijing
- 专利权人： BEIJING ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES,Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
- 当前专利权人： BEIJING ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES,Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
- 当前专利权人地址： CN Beijing; CN Beijing
- 优先权： CN201710514085.9 20170629
- 国际申请： PCT/CN2017/111859 WO 20171120
- 主分类号： C12N15/82
- IPC分类号： C12N15/82 ; C12Q1/686 ; C12Q1/6895
摘要：
The present invention uses the CRISPR/Cas9 genome editing technology to rapidly create a male sterile line of tomato and application thereof, The present invention uses the CRISPR/Cas9 genome editing technology to edit the Solyc03g053130 gene of tomato, and then obtains a male sterile mutant which is homozygous and does not contain the CAS9 transgene by self-crossing. The present invention also discloses a method for assisting in identification of a male sterile plant, which is to detect the genotype of SNP1606 in the Solyc03g053130 gene in the genome of tomato, and if the genotype of SNP1606 of the genome of the tomato to be tested is homozygous T/T, the tomato to be tested is a male sterile plant or is a candidate male sterile plant. The male sterile line of tomato and the method for detecting male sterile plants created by the present invention can be applied to other tomato strains, and have great application prospect and economic value in breeding.
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