A display substrate and a manufacturing method therefor, and a display device are provided. The display substrate includes a base substrate, a plurality of sub-pixels, a plurality of data lines, a plurality of data lead lines, at least one group of contact pads, and a first insulation layer. The base substrate includes a display region and a bonding region; the data lines are configured to provide data signals to the sub-pixels; the data lead lines are electrically connected to the data lines, respectively; the at least one group of contact pads includes a first group of contact pads and a second group of contact pads; the first insulation layer is in gaps between the contact pads and covers edges of the contact pads.