- 专利标题: METHOD FOR MEASURING CELLULAR UPTAKE OF MOLECULES
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申请号: US16756415申请日: 2018-10-19
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公开(公告)号: US20210190764A1公开(公告)日: 2021-06-24
- 发明人: Yuki NOGUCHI , Takeru NAMBU , Kazuhisa OZEKI , Noriaki OHMINATO , Toshito NAKAGAWA , Sotaro NAOI
- 申请人: Chugai Seiyaku Kabushiki Kaisha
- 申请人地址: JP Tokyo
- 专利权人: Chugai Seiyaku Kabushiki Kaisha
- 当前专利权人: Chugai Seiyaku Kabushiki Kaisha
- 当前专利权人地址: JP Tokyo
- 优先权: JP2017-203994 20171020
- 国际申请: PCT/JP2018/039005 WO 20181019
- 主分类号: G01N33/50
- IPC分类号: G01N33/50
摘要:
The present invention provides a method for measuring a cellular uptake amount of a molecule, comprising (i) adding the molecule to an organ-derived cell population to perform incubation, (ii) sorting the organ-derived cell population based on the expression levels of CD31 and CD45, and (iii) after steps (i) and (ii), measuring the amount of the molecule incorporated into the cell population sorted in the step (ii), wherein the molecule is incorporated into cells via a cell surface receptor.
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