A testicular volume measuring device has a container, a lid, a sealing ring, and a plurality of indicia. The lid is removably attached atop the container, and the plurality of indicia is positioned on a lateral wall of the container, which is constructed of a transparent material and delineates an internal cavity. The lid has multiple lid members, which are removably attached to each other. The lid members have cutouts which form a central aperture on the lid, within which the sealing ring is secured during use. One or more testicles may be placed through the sealing ring, around which the lid members are affixed, and the lid is attached atop the container. Fluid volume measurements using the indicia before and after placement of the testicles into a quantity of fluid contained within the cavity enable measurement of the volume of the testicles through fluid displacement.