The present disclosure provides a backlight module, a method for preparing the same, a driving method and a display device. The backlight module includes a backlight source and a light guide structure arranged on a light emitting surface of the backlight source; the backlight source includes a substrate and a plurality of light emitting units arranged on the substrate at intervals; the light guide structure includes a first medium layer and a second medium layer, the first medium layer includes a plurality of first medium structures corresponding to the plurality of light emitting units respectively, and an orthogonal projection of each light emitting unit on the substrate is located within an orthogonal projection of a corresponding first medium structure on the substrate; and the second medium layer includes a plurality of second medium structures, the plurality of second medium structures and the plurality of first medium structures are arranged alternately.