The present application discloses a method, device and system for data transmission, and a display device, and belongs to the field of manufacturing of a liquid crystal panel. The method is applied to a controller. The controller is connected to a plurality of driver chips connected in parallel via a first signal line, and the plurality of driver chips is sequenced according to a response feedback sequence. The method comprises: generating a data request instruction; sending, via the first signal line, a data request instruction; receiving, via the first signal line, the data response instructions sequentially sent by the plurality of driver chips according to the response feedback sequence, wherein each of the data response instructions comprises an identity of a corresponding driver chip and data of the corresponding driver chip. The present application may enrich the function of the first signal line and improve the utilization rate.