Embodiments of the present disclosure provide a display substrate, a display panel, a display device, and a manufacturing method of the display substrate. The display substrate includes a display region and a peripheral region located at an outer side of the display region, and the peripheral region includes a bonding region. The display substrate includes: a base substrate, and a first metal pattern and a second metal pattern which are provided on the base substrate and located in the bonding region, the second metal pattern covers at least a portion of at least one side surface of the first metal pattern, and an activity of a metal of the second metal pattern is weaker than an activity of a metal of the first metal pattern.