The present disclosure provides a method and a device for determining a pixel compensation value, an electronic device and a storage medium. The method includes: enabling a pixel to display at three test gray scales respectively, and obtaining an actual luminosity of the pixel at each test gray scale; calculating compensation values of the pixel at the three test gray scales according to actual luminosities and standard luminosities of the pixel at the three test gray scales respectively, wherein the compensation value of the pixel at any gray scale is a gray scale which can enable the actual luminosity of the pixel to be equal to the standard luminosity of the pixel at the gray scale; calculating standard coefficients Gain1, Gain2 and Offset according to a specific formula; and calculating the compensation value y of the pixel at the gray scale x according to a specific formula.