The porous microwell according to an exemplary embodiment of the present invention is characterized in that as a porous microwell which is a cell culture surface for culturing cells, at least one thereof is indented in a downward direction and is located within a region formed by a penetrating part of a cell culture vessel, and the porous microwell includes a plurality of pores together with a connection part formed therearound, and a first porosity formed by first pores thereof and a second porosity formed by second pores formed in the connection part are different from each other.