The present disclosure relates to a display substrate, a display panel, a display device, and a manufacturing method of the display panel. The display substrate display substrate includes a display area, the display area including: a plurality of sides, wherein adjacent sides of the plurality of sides intersect to form a plurality of corners; a first display area having a first pixel density; and a second display area having a second pixel density lower than the first pixel density, wherein the second display area is located in at least one of the corners.