A method of controlling a driving circuit applicable to a light-emitting substrate which includes a light-emitting assembly including a plurality of light-emitting-element strings connected in parallel, each of the plurality of light-emitting strings comprising a plurality of light-emitting-elements connected in series, the driving circuit connected to the plurality of light-emitting-element strings via a wiring, wherein the method comprises: obtaining a wiring IR drop, a light-emitting element string IR drop, a voltage deviation, and a channel IR drop; obtaining a target power supply voltage according to the wiring IR drop. the light-emitting element string IR drop, the voltage deviation, and the channel IR drop; comparing the target power supply voltage with an output voltage to obtain a comparison result; generating an adjustment signal according to the comparison result; and adjusting the output voltage according to the adjustment signal. A driving circuit and a light-emitting substrate are further provided.