A display substrate, a manufacturing method thereof and a display apparatus are provided. The display substrate includes a display region and a bezel region. The display region includes a plurality of sub-pixels, and the display region includes a main display region and a plurality of corner display regions. The corner display region includes a first display region and a second display region, a distance between a center point of the first display region and a center point of the main display region is smaller than a distance between a center point of the second display region and the center point of the main display region; and a structure of sub-pixels of the first display region is different from a structure of sub-pixels of the second display region.