A system and a method of calculating coordinates of a pupil center point are provided. The system for acquiring the coordinates of the pupil center point includes a first camera, a second camera, a storage and a processor. The first camera is configured to capture a first image including a face and output the first image to the processor, the second camera is configured to capture a second image including a pupil and output the second image to the processor, a resolution of the first camera is smaller than a resolution of the second camera, and the storage is configured to store processing data, and the processor is configured to: acquire the first image and the second image; extract a first eye region corresponding to an eye from the first image; convert the first eye region into the second image, to acquire a second eye region corresponding to the eye in the second image; and detect a pupil in the second eye region and acquire the coordinates of the pupil center point.