The present disclosure provides a system including an insulator-based dielectrophoresis device. The insulator-based dielectrophoresis device includes a fluid flow channel having at least one fluid inlet and at least one fluid outlet. The fluid flow channel includes at least one insulating flow structure extending from a wall to define a constriction in the fluid flow channel. The system includes a detection chamber placed in fluid communication with the fluid flow channel by an opening in the wall of the fluid flow channel, where the opening is configured downstream of the at least one insulation flow structure. The detection chamber includes an electrochemical sensor configured to constrict the flow of fluid entering the detection chamber through a pore, where the pore is sized to produce a detectable signal upon passage of an analyte through the pore. The system may process the detectable signal to output a metric indicative of the identity or physiochemical property of the analyte.