Disclosed are a display panel, a manufacturing method thereof and a displaying device. The display panel comprises a driving backplane, a light-emitting device layer disposed on the driving backplane, and an encapsulation layer, a color film layer and a color separation suppression layer which are disposed on the side, away from the driving backplane, of the light-emitting device layer, wherein the color separation suppression layer is configured to interfere with and cancel out external ambient light, so as to reduce the external ambient light incident on the light-emitting device layer and the driving backplane and block the external ambient light reflected by the light-emitting device layer and the driving backplane from exiting from a light-exiting surface of the display panel.