A guiding system and a guiding method for ultrasound scanning operation are provided. The guiding system includes a handheld guiding device, a display device, an ultrasound scanning device, a prompting device, and a control host. When the handheld guiding device generates a first physical motion, the control host detects the first physical motion and generates navigation prompting information accordingly. The prompting device is suitable for presenting the navigation prompting information to guide the ultrasound scanning device to move to generate a second physical motion. The control host captures an ultrasound image via the ultrasound scanning device and sends the ultrasound image to the display device at a guiding end for display.