Provided are a display substrate, a tiled display panel and a display device, relating to the field of display technology. The display substrate comprises a base substrate, and a plurality of light-emitting units, a protective layer and a connecting wire which are sequentially stacked in a direction away from the base substrate. One end of the connecting wire is connected to the plurality of light-emitting units through a via hole provided in the protective layer, and the other end is used for connecting a drive circuit, that is, the drive circuit can be directly provided at a side of the protective layer away from the base substrate. When a plurality of display substrates are tiled to form a tiled display panel, there is no need to bend a flexible circuit board at sides of each display substrate, and therefore, the gap between every two adjacent display substrates is small, and the display effect is good.