A light-emitting device and an electronic apparatus including the light-emitting device. The light-emitting device includes: a first electrode and a second electrode each having a surface opposite the other; and an interlayer disposed between the first electrode and the second electrode, wherein the interlayer includes an emission layer and a hole transport region, the hole transport region is disposed between the first electrode and the emission layer. The emission layer includes a first emission layer and a second emission layer, the first emission layer is disposed between the hole transport region and the second emission layer, wherein the first emission layer includes a first host and a first light-emitting material, and the second emission layer includes a second host and a second light-emitting material. The second host is a substituted anthracene compound, and the first host and the second host are different from each other. A lowest unoccupied molecular orbital (LUMO) energy level of the second host is less than a LUMO energy level of the first host, and each of the LUMO energy level of the first host and the LUMO energy level of the second host has a negative value and is determined using a density functional theory (DFT) method.