A locker with a valve assembly includes a locker body and an air supply assembly disposed on the locker body. A main air duct communicating with the air supply assembly is disposed inside the locker body. The locker body includes at least one storage box. The storage box includes a box body, a box door and a valve assembly. The box body includes a sidewall that surrounds a circle and further includes an inner wall connected to the rear end of the sidewall. The front end of the sidewall forms an opening. The sidewall is provided with an air vent communicating with the main air duct. The box door has a closed position for closing the opening, and an open position for opening the opening. The valve assembly includes a valve and a reset member. The valve is configured to move relative to the sidewall.