Provided are a display substrate and a preparation method therefor, and a display apparatus, which can not only avoid jumpers generated when a light-emitting device in a sub-pixel is electrically connected to a storage capacitor, in order to avoid the risk of crosstalk, but can also increase the pixel aperture ratio of the display substrate and increase the capacitance of the storage capacitor in a pixel driving circuit. The display substrate comprises: a base, and a pixel driving circuit and a bottom-emission-type light-emitting device which are arranged in a display region on the base and are located in each sub-pixel, wherein the light-emitting device comprises a first electrode connected to the pixel driving circuit; the pixel driving circuit comprises a first storage capacitor; the first storage capacitor comprises a first storage electrode and a second storage electrode which are arranged opposite each other; the first electrode is multiplexed as the first storage electrode; and the second storage electrode and the first electrode are both transparent electrodes.