An array substrate, a manufacturing method thereof and a display panel are provided. The array substrate includes sub-pixel units, and each sub-pixel unit includes a light emitting region and a non-light emitting region; each sub-pixel unit includes a light emitting element, the light emitting element includes a light emitting layer and a first electrode, and at least a part of the first electrode is in the light emitting region. A plurality of first wires are configured to supply a power signal to the light emitting element and include a first sub-wire; the first sub-wire includes a plurality of portions, adjacent two of the plurality of portions are spaced apart from each other by an opening in the light emitting region; at least a part of an orthographic projection of the opening on the array substrate does not overlap with an orthographic projection of the first electrode on the array substrate.