Provided are a display substrate, an organic light-emitting display panel, and a display device. A shielding layer is arranged between a first gate metal layer and a source-drain metal layer such that same are insulated from each other, an orthographic projection, on a base substrate, of the shielding layer covers at least an orthographic projection, on the base substrate, of an overlapping area of a scanning signal line and a detection signal line, and the shielding layer is coupled to a fixed voltage.