The present disclosure relates to an antibacterial glass composition and a manufacturing method thereof. The antibacterial glass composition according to the present disclosure comprises: 20-45 wt % of SiO2; 1-30 wt % of B2O3; 10-30 wt % of ZnO; 5-25 wt % of one or more of Na2O, K2O and Li2O; 0.1-10 wt % of one or more of Al2O3 and TiO2; 0.1-5 wt % of NaF; and 1-10 wt % of one or more of Co3O4, CuO and Fe2O3, thereby causing no deterioration in durability and ensuring excellent antibacterial activity.