A display substrate and a manufacture method thereof, and a display device are provided. The display substrate includes a display region and a peripheral region, the peripheral region includes a first scan driving circuit and a second scan driving circuit on a first side of the display region, the peripheral region further includes a binding region on a second side of the display region, the peripheral region includes an organic insulation layer, the organic insulation layer at least partially covers the first scan driving circuit and the second scan driving circuit, and includes a first groove that is in a strip shape and extends substantially along a first direction to expose a portion between the first scan driving circuit and the second scan driving circuit, and the first groove also extends from the first side to the second side and extends substantially along a second direction on the second side.