Disclosed in embodiments of the present disclosure are a display substrate, a display panel and a display device. The display substrate includes a plurality of first light-emitting devices in a first display area, a plurality of second light-emitting devices in a second display area, a plurality of first pixel driving circuits in the second display area, the first pixel driving circuits are connected with the first light-emitting devices, and the plurality of pixel driving circuit has a drive transistor; gate connecting electrodes connected to a gate electrode of the drive transistor; a plurality of traces, each of at least part of the traces being electrically connected from the first pixel driving circuits across the gate connecting electrodes to the first light-emitting devices; and a shielding layer including isolation parts, an orthographic projection of the isolation parts at least partially overlapping an orthographic projection of the gate connecting electrodes.