A display substrate, a display panel and a manufacturing method thereof, and a display device are disclosed. The display substrate includes: a first substrate; a pixel defining layer on the first substrate and having pixel openings; and light emitting devices in one-to-one correspondence with the pixel openings. The light emitting device includes a first electrode and a light emitting function layer, the first electrode is between the pixel defining layer and the first substrate, and includes a main body portion exposed by the pixel opening; the light emitting function layer is in the pixel opening and on a side of the main body portion away from the first substrate, at least a part of the light emitting function layer directly faces the main body portion, and the part of the light emitting function layer directly facing the main body portion is a curved film layer protruding towards the first substrate.