A display substrate has a display area and an encapsulation area around the display area. The display substrate includes a substrate and a spacer layer. The spacer layer is located on a side of the substrate, and includes a plurality of first columnar spacers located in the encapsulation area and distributed at intervals around the display area, and bottom ends of the plurality of first columnar spacers are fixedly disposed on the substrate. Ends of the plurality of first columnar spacers proximate to the substrate are the bottom ends thereof, and ends of the plurality of first columnar spacers away from the substrate are top ends thereof.