The present disclosure relates to a microcontroller, a readout integrated circuit and a method of driving a circuit, and more particularly, to a microcontroller, a readout integrated circuit and a method of driving a circuit to transmit command signals by using a pair of lines, through which signals are transmitted from the microcontroller which is a master circuit to the readout integrated circuit which is a slave circuit, as two single-ended signal transmission lines and to control power of the readout integrated circuit which is a slave circuit.