A display device, a display panel, and a manufacturing method thereof are provided. The display panel includes: a display substrate comprising a display region, wherein an opening region and an isolation region surrounding the opening region are arranged within the display region; a light-emitting layer formed on the display region; a blocking layer formed on a surface of the light-emitting layer away from the display substrate; and a first packaging layer covering the light-emitting layer and the blocking layer, wherein the first packaging layer is patterned through a photoresist layer to form a first through hole on the first packaging layer; a second through hole is respectively formed at a part of the blocking layer corresponding to the first through hole and at a part of the light-emitting layer corresponding to the first through hole.