A fluid handling device includes a sample channel configured to carry a sample; a dispersion medium channel configured to carry dispersion medium; a dispersion liquid generation part connected to the sample channel and the dispersion medium channel, and configured to generate dispersion liquid by dividing the sample by the dispersion medium, the dispersion liquid being liquid in which droplets of the sample are dispersed in the dispersion medium; and a dispersion liquid channel connected to the dispersion liquid generation part. The dispersion liquid generation part includes a protrusion.