Disclosed are a display substrate and a manufacturing method therefor, and a display panel and a display device. The arrangement of a first isolation portion and a second isolation portion at least disconnects a first isolated portion from one of a second isolated portion and a third isolated portion; therefore, the effect of isolating a cathode can be enhanced when an isolated layer is the cathode multiplexed into a self-capacitance electrode; and when the isolated layer is an inorganic film encapsulation layer contained in a bezel area, cracks generated by cutting or for other reasons may be prevented from extending to a display area.