Embodiments of the present disclosure provide a display substrate, a display device and a mask, belonging to the field of display technology. The display substrate in the embodiments of the present disclosure has a stretchable area including an opening region, a bridge region and an island region. The display substrate includes: a base substrate; a plurality of light-emitting units, each light-emitting unit including a light-emitting device of at least one color; each island region is provided with at least one light-emitting unit. When the stretchable area is not stretched, light-emitting centers of the plurality of light-emitting units are arranged in a non-uniform mode, and after the stretchable area is stretched, the light-emitting centers of the light-emitting units are arranged in a uniform mode.