Barcode readers and barcode reader assemblies with lift handles are disclosed herein. An example bioptic barcode reader assembly includes a barcode reader having a housing and a metal frame secured to the housing. A retractable first lift handle having an elongated first aperture is positioned on a first side of the housing and a retractable second lift handle having an elongated second aperture is positioned on a second side of the housing, opposite the first side. Each of the first and second lift handles is movable relative to the housing between a retracted position and an extended position and the first and second apertures in the first and second lift handles overlie a first center of gravity of the barcode reader and a second center of gravity of the barcode reader assembly.