Disclosed are a display substrate (10) and a display device. The display substrate (10) includes at least one irregularly-shaped pixel (103), and a shape of a boundary line of a side, proximal to a peripheral region (101b), of each irregularly-shaped pixel (103) matches with a shape of a boundary line (101a1) of an irregularly-shaped display region (101a) in a base substrate (101), such that the irregularly-shaped pixel (103) does not go beyond the irregularly-shaped display region (101a) of the display substrate (10), a narrow bezel of the display substrate (10) can be realized conveniently, and an image displayed at the boundary line (101a1) of the irregularly-shaped display region (101a) may be prevented from being in a zigzag shape, thereby ensuring a display effect of the display device. Moreover, because an area of an orthographic projection of the irregularly-shaped pixel (103) on the base substrate (101) is smaller than an area of an orthographic projection of a rectangular pixel (102) on the base substrate (101), an area of an opening (a) formed by a black matrix layer (104) in a region where each rectangular pixel (102) is disposed is larger than an area of an opening (b) formed by the black matrix layer (104) in a region where any irregularly-shaped pixel (103) is disposed, such that a smooth transition of luminance of light emitted by the irregularly-shaped pixel (103) and the rectangular pixel (102) can be ensured, and the luminance uniformity of the display device is better.